Blast Crisis in Chronic Myeloid Leukemia: An Immunophenotypic Analysis
Singh Ak.1, Singh An.1, Kanaujia S.1, Kushwaha R.1, Singh U. S.1
1 King George’s Medical University, Lucknow, India


Background and Aim of Study: There are two different phases of untreated chronic myeloid leukemia – chronic phase, and blast crisis – according to the World Health Organization classification of Hematolymphoid tumors. The blast cells in the chronic myeloid leukemia blast crisis can express myeloid, lymphoid, bi-phenotypic, monocytic, megakaryocytic, and erythroid phenotypes. The immunophenotype of blast population determines how chronic myeloid leukemia – blast crisis patients respond to treatment, hence flowcytometric examination is required.
The aim of the study: to assess immunophenotyping outcomes of flowcytometry performed on the chronic myeloid leukemia – blast crisis.
Material and Methods: A five-year retrospective descriptive analysis was carried out in Pathology Department at King George’s Medical University Lucknow, India (2017-2021). The patient’s peripheral blood and bone marrow aspirate samples were analyzed. Clinical, hematological, and immunophenotypic data were retrieved. The flow cytometry samples were prepared using the standardized “lyse-stain-wash” method.
Results: A total of 43 cases of chronic myeloid leukemia – blast crisis were retrieved from the departmental archive in 5 years. The mean age of study population was 39.62±14.86 years. There were 24 males and 19 females. 27 patients were diagnosed with myeloid blast crisis, 14 cases of B-lymphoid blast crisis and 2 cases of mixed phenotypic acute leukemia.
Conclusions: Identification of the blast lineage of patients with chronic myeloid leukemia – blast crisis is crucial since the existence of atypical blast phenotypes influences the disease treatment and prognosis.



immunophenotyping, flowcytometry, chronic myeloid leukemia, lymphoid blast, myeloid blast, mixed phenotypic acute leukemia



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Information about the author:

Singh Akanksha (Corresponding Author) – https://orcid.org/0000-0001-5398-6203; This email address is being protected from spambots. You need JavaScript enabled to view it., MD, Senior Resident, Department of Pathology, King George’s Medical University, Lucknow, India.

Singh Anuraghttps://orcid.org/0000-0002-1979-8847; MD, Senior Resident, Department of Pathology, King George’s Medical University, Lucknow, India.

Kanaujia Swetahttps://orcid.org/0000-0003-3369-1664; MD, Resident, Department of Pathology, King George’s Medical University, Lucknow, India.

Kushwaha Rashmi https://orcid.org/0000-0002-0581-2177; MD, Professor, Department of Pathology, King George’s Medical University, Lucknow, India.

Singh Uma Shankarhttps://orcid.org/0000-0002-9748-9484; MD, Professor, Head of the Department of Pathology, King George’s Medical University, Lucknow, India.

Cite this article as:


Singh, Ak., Singh, An., Kanaujia, S., Kushwaha, R., & Singh, U. S. (2022). Blast crisis in chronic myeloid leukemia: An immunophenotypic analysis. International Journal of Science Annals, 5(1-2), 31–37. https://doi.org/10.26697/ijsa.2022.1-2.3


Singh, Ak., Singh, An., Kanaujia, S., Kushwaha, R., & Singh, U. S.. 2022. "Blast crisis in chronic myeloid leukemia: An immunophenotypic analysis". International Journal of Science Annals, [online] 5(1-2), pp. 31-37. viewed 25 December 2022, https://culturehealth.org/ijsa_archive/ijsa.2022.1-2.3.pdf


Singh Ak., Singh An., Kanaujia S., Kushwaha R., & Singh U. S. Blast crisis in chronic myeloid leukemia: An immunophenotypic analysis. International Journal of Science Annals [Internet]. 2022 [cited 25 December 2022]; 5(1-2): 31-37. Available from: https://culturehealth.org/ijsa_archive/ijsa.2022.1-2.3.pdf https://doi.org/10.26697/ijsa.2022.1-2.3

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